E-GEOD-40282 - Gene expression data of Lsd1fl/fl and Lsd1fl/fl Mx1Cre Gr1dim Mac1 granulocytic progenitor cells
Released on 25 June 2013, last updated on 3 July 2015
We discovered that mice with hematopoietic-specific deletion of Lsd1 lacked Gr-1+ Mac1+ neutrophilic granulocytes whereas the numbers of Gr-1dim Mac1+ granulocytic progenitor cells was increased. To determine the genes altered by Lsd1-loss, Gr-1dim Mac1+ granulocytic progenitor cells from Lsd1fl/fl and Lsd1fl/fl Mx1Cre mice were FACS-purified to be analyzed by gene expression profiling. Primary Gr-1dim Mac1+ granulocytic progenitor cells were isolated from the bone marrow of Lsd1fl/fl and Lsd1fl/fl Mx1Cre animals by FACS-sorting, one week after the final p(I:C) dose. Total RNA from three biological replicates per genotype was extracted and used to hybridize to Affymetrix expression arrays using the Mouse Genome 430 2.0 array platform.
transcription profiling by array
Marc A. Kerenyi <email@example.com>, Marc A Kerenyi, Stuart H Orkin
Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. Kerenyi MA, Shao Z, Hsu YJ, Guo G, Luc S, O'Brien K, Fujiwara Y, Peng C, Nguyen M, Orkin SH. , Europe PMC 23795291