Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-40141 - α-catulin play a role in lung cancer metastasis

Status
Released on 16 August 2012, last updated on 3 May 2014
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (7)
Description
Background: α-catulin may functions as an oncoprotein, sustaining proliferation by preventing cellular senescence and promoting cancer cell migration. In this study, we investigated the mechanism of α-catulin in cancer cell migration and metastasis in lung cancer. Method: α-catulin mRNA expression was isolated from A549/AS2neo (control) and A549/AS2neo-α-catulin stable cells. The Phalanx Human OneArray microarray analysis was performed to identify α-catulin downstream genes. Results: Overexpression of α-catulin increased cancer cell migration and metastasis. By using Phalanx Human OneArray microarray we have identified panel of genes altered by α-catulin overexpression, consisting of CDC42, intergrins and genes related to cytoskeleton remodeling. Conclusion: α-catulin is an oncoprotein and promotes lung cancer cell migration and metastasis. Two-condition experiment, Vector vs. alpha-catulin overexpression cells.The cDNAs encoding full-length human alpha-catulin were amplified and subcloned into lentiviral pLKO_AS2.neo which generated full-length alpha-catulin. Vector control or alpha-catulin lentivirus were transduced into A549 cells and G418 was used to select stable cells.
Experiment type
transcription profiling by array 
Contacts
Tse-Ming Hong <htmjimmy@gmail.com>, C H Liang, T M Hong, Y L Chen
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40141.idf.txt
Sample and data relationshipE-GEOD-40141.sdrf.txt
Raw data (1)E-GEOD-40141.raw.1.zip
Processed data (1)E-GEOD-40141.processed.1.zip
Additional data (1)E-GEOD-40141.additional.1.zip
Array designA-GEOD-13287.adf.txt
R ExpressionSetE-GEOD-40141.eSet.r
Links