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E-GEOD-40100 - Expression data of spleens from chickens infected with different Newcastle Disease Viruses

Status
Released on 14 August 2012, last updated on 20 August 2012
Organism
Gallus gallus
Samples (9)
Array (1)
Protocols (6)
Description
Genotype VIId NDV is characterized by severe tissue damage in chicken lymphoid organs compared to other virulent strains. However, biological basis of this unusual pathological phenotype is unknown. Host response is associated with pathogenicity of Newcastle Disease Virus (NDV). We aim to determine the contribution of host response to the severe tissue destruction in the lymphoid system caused by genotype VIId NDV. We used microarray analysis to evaluate the global transcriptional response in the spleen of chickens infected with genotype VIId NDV strain JS5/05 and genotype IV NDV Herts/33. Chickens were inoculated with JS5/05 or Herts/33 or mock-infected. At day 2 post infection, spleens were isolated from three chickens per group for RNA extraction and hybridization on Affymetrix microarrays. Samples were named as follows: JS5/05 (I4_1_NS,I4_2_NS,I4_3_NS), Herts/33 (H1_NS,H2_NS, H3_NS), control (C1_NS, C2_NS, C3_NS).
Experiment type
transcription profiling by array 
Contacts
Jiao Hu, Zenglei Hu
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40100.idf.txt
Sample and data relationshipE-GEOD-40100.sdrf.txt
Raw data (1)E-GEOD-40100.raw.1.zip
Processed data (1)E-GEOD-40100.processed.1.zip
Array designA-AFFY-103.adf.txt
R ExpressionSetE-GEOD-40100.eSet.r
Links