Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-40028 - The genomic foundation of heterotrophy to photoautotrophy transition in Oleaginous Chlorella

Status
Released on 2 November 2015, last updated on 24 November 2015
Organism
Chlorella pyrenoidosa
Samples (18)
Protocols (4)
Description
The understanding of molecular events occurring in Chlorella during heterotrophy to photoautotrophy transition as well as sudden light stress and glucose starvation, are still largely unknown. To well grasp its cellular metabolism, particularly the regulation of biosynthesis and degradation pathways of lipid, protein and carbohydrates, as well as the diverse trophic adaptation affecting carbon partitioning during heterotrophy to photoautotrophy transition process, we sequenced the transcriptome (RNA-seq) in six time points to discover how transcriptional changes in C. pyrenoidosa modulate metabolic flux trends leading to intracellular components dynamic reassortment. Three independent biological experiments (biological replicates) were carried out in the same batch. In total, eighteen samples collected at six time points (0-, 24-, 72-h from heterotrophic cells and 2-, 8-, 24-h from phototrophic cells) were used for mRNA-Seq library preparation and then submitted to Solexa GA-IIx (Illumina, USA) for sequencing.
Experiment type
RNA-seq of coding RNA 
Contacts
Jianhua Fan <jhfanster@gmail.com>, Jian Xu, Kang Ning, Yuanguang Li
Citation
Genomic Foundation of Starch to Lipid Switch in Oleaginous Chlorella. Fan J, Ning K, Zeng X, Luo Y, Wang D, Hu J, Li J, Xu H, Huang J, Wan M, Wang W, Zhang D, Shen G, Run C, Liao J, Fang L, Huang S, Jing X, Su X, Wang A, Bai L, Hu ZM, Xu J, Li Y. , PMID:26486592
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-40028.idf.txt
Sample and data relationshipE-GEOD-40028.sdrf.txt
Additional data (1)E-GEOD-40028.additional.1.zip
Links