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E-GEOD-40023 - Diurnal Gene Variation in Mouse Eye

Status
Released on 9 August 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (48)
Array (1)
Protocols (5)
Description
The purpose of this study was to explore diurnal gene expression changes that occur in the RPE/Choroid/Sclera. Mice C57BL/6J were purchased from the Jackson Laboratory (Bar Harbor, ME) and raised to approximately 2 months of age and entrained to a 12hr/12hr light/dark cycle for two weeks. Eye cups from male mice were rapidly dissected and RPE/ choroid/sclera tissues were collected over three consecutive diurnal cycles at Zeitgeber time (ZT) 0.5, 1, 1.5, 4, 11, 13, 16, and 23 hrs, for a total of 24 time points. Three mice were used for each time point and dissections for dark time points were done under dim-red light. To generate a reference RNA for microarray hybridization, whole eyes from equal numbers of male and female mice were collected at ZT6 and ZT7. Time: Samples were collected at the indicated Zeitgeber time points time series design
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40023.idf.txt
Sample and data relationshipE-GEOD-40023.sdrf.txt
Processed data (1)E-GEOD-40023.processed.1.zip
Array designA-GEOD-9214.adf.txt
Links