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E-GEOD-39989 - Transcription profiling by array of Olfm4-deficient murine prostate

Status
Released on 6 July 2016, last updated on 31 October 2016
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (7)
Description
Purpose: To explore the mechanisms underlying increased tumorigenesis of Olfm4-deficient murine prostate. Experiment design: Comparison of gene expression between Olfm4-knockout and wild-type mice at 3 months of age in the prostate tissue. Experimental procedures: Total RNA was purified from whole prostate tissue of Olfm4 (+/+) and Olfm4 (-/-) mice at 3 months of age using the RNeasy Plus Mini Kit (Qiagen). Microarray analyses were performed by the NIDDK Core Facility at the National Institutes of Health using Affymetrix Mouse Genome 430 2.0 Array GeneChips (Affymetrix; Santa Clara, CA). Biological replications were used for the Olfm4 (+/+) or Olfm4 (-/-) prostate RNA extracted from five individual mice. The microarray signals were analyzed using the Affymetrix RMA algorithm. The analysis of variance results, False Discovery Rate (FDR) reports, and heatmaps were generated using Partek Genomic of tware 6.5 (Partek; St. Charles, MO). Pathway analyses were performed using MetaCore webaccess software (http://www.genego.com/). Comparison of KO vs. WT with four replications per treatment sample
Experiment type
transcription profiling by array 
Contacts
WeiPing Chen <weipingChen@niddk.nih.gov>, Chu-Xia Deng, Griffin P Rodgers, Hongzhen Li, Jianqiong Zhu, Weiping Chen, Wenli Liu
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39989.idf.txt
Sample and data relationshipE-GEOD-39989.sdrf.txt
Raw data (1)E-GEOD-39989.raw.1.zip
Processed data (1)E-GEOD-39989.processed.1.zip
Array designA-AFFY-45.adf.txt
Links