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E-GEOD-39843 - Expression data of cystic fibrosis and non-cystic fibrosis airway cell lines under oxidative stress

Status
Released on 2 August 2012, last updated on 30 September 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (8)
Description
CF's physiopathology is poorly explained by the mutation alone. The oxydative stress could be a major factor of this illness . Study its impact on transcriptome's CF cell line could be ameliorate our understanding of the evolution of cystic fibrosis. we used microarray technology to evaluate under oxydative stress, the transcriptional state of an epithelial lung cell issued from a human with cystic fibrosis and to identify a set of modulated genes associated to survival cell processes. the two cell lines are cultivated to Air-liquid Interface for RNA extraction and hybridization on Affymetrix microarrays. Each condition is triplicated. For the oxidative stress conditions, the two cell lines are treated on apical site by 15 µl of DMNQ (2,3-dimethoxy-1,4-naphtoquinone) ,concentrated at 15 µM, during 24 hours before RNA extraction.
Experiment type
transcription profiling by array 
Contacts
greg Voisin <voisingreg@yahoo.fr>, G Voisin, Y Berthiaume
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39843.idf.txt
Sample and data relationshipE-GEOD-39843.sdrf.txt
Raw data (1)E-GEOD-39843.raw.1.zip
Processed data (1)E-GEOD-39843.processed.1.zip
Array designA-AFFY-44.adf.txt
Links