E-GEOD-39438 - Transcriptomic profiling of FAP+ fibroblasts
Released on 15 July 2013, last updated on 5 September 2013
FAP+ cells had been reported to be present only in healing wounds and at sites of tissue remodelling. We have found FAP+ cells in many normal tissues and aimed to investigate whether these cells were related or whether FAP is upregulated in response to an external stimulus on a variety of cells. Furthermore we wanted to investigate whether FAP+ cells are simply fibroblasts and so compared their transcriptomic profiles to that of a typical fibroblastic population, the murine embryonic fibroblast. FAP+ cells were sorted from two mesenchymal tissues, visceral adipose and skeletal muscle, and from an epithelial organ, the pancreas. These were compared to MEFs. Cells were isolated in duplicate experiments and these were analysed separately. These were compared to previously published publically available CD4+ T-cell subset data.
RNA-seq of coding RNA
Andrew Deonarine <firstname.lastname@example.org>, A Deonarine, B Ren, C Feig, D T Fearon, E W Roberts, J O Jones, O Caballero, S Teichmann