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E-GEOD-39346 - The oncoprotein v-Myb activates transcription of Gremlin 2 during differentiation of the chicken neural crest to melanoblasts

Status
Released on 13 July 2012, last updated on 23 July 2012
Organism
Gallus gallus
Samples (6)
Array (1)
Protocols (8)
Description
The neural crest (NC) is a transient dynamic structure of ectodermal origin, found in early vertebrate embryos. The multipotential NC cells migrate along well defined routes, differentiate to various cells types including melanocytes and participate in the formation of various permanent tissues. Abnormal development of NC cells causes several human diseases – neurocristopathies. As there is only limited information about the molecular mechanisms controlling early events in melanocyte specification and development, we exploited the AMV v-Myb transcriptional regulator, which directs differentiation of in vitro chicken NC cells to the melanocyte lineage. This activity is strictly dependent on v-Myb specifically binding to the Myb recognition DNA element (MRE). The two tamoxifen-inducible v-myb alleles were constructed, one which recognizes the MRE and one which does not. These were activated in ex-ovo NC cells, and the expression profiles of resulting cells were analyzed using Affymetrix microarrays and RT-PCR. These approaches revealed up-regulation of the BMP antagonist gremlin 2 mRNA, and down-regulation of mRNAs encoding several epithelial genes including KRT19 as very early events following the activation of melanocyte differentiation by v-Myb. Comparison of gene expression profiles of chicken neural crest cells constitutively expressing 4-OH-tamoxifen inducible v-myb with mutated leucine zipper region or a version with an additional point mutation (N118D) in the DNA-binding domain. Three biological replicates were analyzed for each group.
Experiment type
transcription profiling by array 
Contacts
Marta Dvorakova, Michaela Starostova, Michal Dvorak, Vit Karafiat, Vladimir Cermak
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39346.idf.txt
Sample and data relationshipE-GEOD-39346.sdrf.txt
Raw data (1)E-GEOD-39346.raw.1.zip
Processed data (1)E-GEOD-39346.processed.1.zip
Array designA-AFFY-103.adf.txt
R ExpressionSetE-GEOD-39346.eSet.r
Links