E-GEOD-38837 - Zbtb20-mediated repression of genes in developing CA1 pyramidal neurons
Released on 11 April 2014, last updated on 2 June 2014
The transcriptional repressor Zbtb20 is essential for specification of hippocampal CA1 pyramidal neurons. Moreover, ectopic expression of Zbtb20 is sufficient to transform subicular and retrosplenial areas of D6/Zbtb20S mice to CA1. We used microarrays to identify genes that are repressed by Zbtb20 in developing CA1 pyramidal neurons in the CA1-transformed cortex of D6/Zbtb20S mice. For RNA extraction and hybridization on Affymetrix microarrays, we isolated the CA1-transformed subiculum and retrosplenial cortex from postnatal day 1 D6/Zbtb20S mice, as well as corresponding areas from their wildtype littermates. Total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (Qiagen). Each RNA sample represents a pool of RNA obtained from dissected tissues of seven animals.
transcription profiling by array
Mads Thomassen <firstname.lastname@example.org>, Jakob V Nielsen, Jens Noraberg, Niels A Jensen, Torben A Kruse
Zbtb20 defines a hippocampal neuronal identity through direct repression of genes that control projection neuron development in the isocortex. Nielsen JV, Thomassen M, Mï¿½llgï¿½rd K, Noraberg J, Jensen NA. , Europe PMC 23283686