E-GEOD-38704 - Regulation of gene expression by RORgamma in Th17 cells
Released on 17 April 2013, last updated on 2 June 2014
We observed significant growth inhibition of melanoma tumor in RORγ-/- mice, suggesting an important role for the T helper 17 cell (TH17) pathway in tumor immunity. Though RORgamma t (RORγt) plays a critical role in the development of IL-17-secreting Th17 cells, the role of other genes remained an open question. To explore the expression of unknown genes with anti-tumor properties, gene expression analysis was performed. Sorted naïve TH cells (CD4+CD25-CD62Lhigh) from RORc+/+ and RORc-/-mice were differentiated under TH17 polarizing conditions. After 4 days, cells were harvested, RNA was isolated and gene expression analysis was performed.
transcription profiling by array
NIEHS Microarray Core <email@example.com>, Anton M Jetten, Hong S Kang, Rahul Purwar, Thomas S Kupper
Robust tumor immunity to melanoma mediated by interleukin-9-producing T cells. Purwar R, Schlapbach C, Xiao S, Kang HS, Elyaman W, Jiang X, Jetten AM, Khoury SJ, Fuhlbrigge RC, Kuchroo VK, Clark RA, Kupper TS. , Europe PMC 22772464