Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-38594 - Genome-wide binding of Yki and GAF in embryos and wing imaginal discs [ChIP-Seq]

Status
Released on 7 February 2013, last updated on 11 March 2013
Organism
Drosophila melanogaster
Samples (14)
Protocols (3)
Description
The Hippo pathway regulates metazoan growth, acting through the transcriptional co-activators Yorkie (in Drosophila) and Yap and Taz (in vertebrates). Much attention has been focused on upstream regulators of Yorkie and its homologues. In contrast, the mechanisms by which they actually promote transcription have remained poorly understood. Genome-wide chromatin binding experiments support extensive functional overlap between Yorkie and GAF. Chromatin binding identifies thousands of Yorkie sites, the majority of which are associated with elevated transcription, based on genome-wide analysis of mRNA and histone H3K4Me3 modification. Our studies establish a molecular basis for transcriptional activation by Yorkie and implicate it as a global regulator of transcriptional activity in Drosophila. This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. This dataset was generated in collaboration with Ken Irvine at HHMI/Rutgers University and Richard S. Mann at Columbia University. It contains ChIP-seq data (Illumina) for multiple transcription factor antibodies in Drosophila embryos and larval wing imaginal discs.
Experiment type
ChIP-seq 
Contacts
Alex Crofts, Hyangyee Oh, Jason Grundstad, Kenneth Irvine, Kevin White, Lijia Ma, Matthew Slattery, Richard Mann
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-38594.idf.txt
Sample and data relationshipE-GEOD-38594.sdrf.txt
Additional data (1)E-GEOD-38594.additional.1.zip
Links