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E-GEOD-3854 - Transcription profiling by array of Drosophila embryos mutant for Ras1, Pnt, lambda-Top, Arm, lmd, Tkv. Dl, wg, Notch, spi, Hti and Dof

Status
Released on 14 August 2007, last updated on 7 November 2011
Organism
Drosophila melanogaster
Samples (54)
Array (1)
Protocols (5)
Description
An important but largely unmet challenge in understanding the mechanisms that govern formation of specific organs is to decipher the complex and dynamic genetic programs exhibited by the diversity of cell types within the tissue of interest. Here, we use an integrated genetic, genomic and computational strategy to comprehensively determine the molecular identities of distinct myoblast subpopulations within the Drosophila embryonic mesoderm at the time that cell fates are initially specified. A compendium of gene expression profiles was generated for primary mesodermal cells purified by flow cytometry from appropriately staged wild-type embryos and from twelve genotypes in which myogenesis was selectively and predictably perturbed. A statistical meta-analysis of these pooled datasets—based on expected trends in gene expression and on the relative contribution of each genotype to the detection of known muscle genes ”provisionally assigned hundreds of differentially expressed genes to particular myoblast subtypes. Whole embryo in situ hybridizations were then used to validate the majority of these predictions, thereby enabling true positive detection rates to be estimated for the microarray data. This combined analysis reveals that myoblasts exhibit much greater gene expression heterogeneity and overall complexity than was previously appreciated. Moreover, it implicates the involvement of large numbers of uncharacterized, differentially expressed genes in myogenic specification and subsequent morphogenesis. These findings also underscore a requirement for considerable regulatory specificity for generating diverse myoblast identities. Finally, to illustrate how the developmental functions of newly identified myoblast genes can be efficiently surveyed, a rapid RNA interference assay that can be scored in living embryos was developed and applied to selected genes. This integrated strategy for examining embryonic gene expression and function provides a substantially expanded framework for further studies of this model developmental system.
Experiment types
transcription profiling by array, cell type comparison, co-expression, individual genetic characteristics
Contact
Citation
An integrated strategy for analyzing the unique developmental programs of different myoblast subtypes. Beatriz Estrada,Sung E Choe,Stephen S Gisselbrecht,Sebastien Michaud,Lakshmi Raj,Brian W Busser,Marc S Halfon,George M Church,Alan M Michelson. , PMID:16482229
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-3854.idf.txt
Sample and data relationshipE-GEOD-3854.sdrf.txt
Raw data (1)E-GEOD-3854.raw.1.zip
Processed data (1)E-GEOD-3854.processed.1.zip
Array designA-AFFY-17.adf.txt
R ExpressionSetE-GEOD-3854.eSet.r
Links