E-GEOD-38409 - Expression data from mouse lungs, exposed in-utero to second-hand smoke (SHS) and challenged with ovalbumin (OVA) as adults

Status
Released on 1 June 2012, last updated on 28 September 2015
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (8)
Description
SHS exposure during pregnancy has adverse effects on offspring. We used microarrays to characterize the gene expression changes caused by in-utero SHS exposure and adult (19-23 weeks) OVA challenge in 23-week mouse lungs. Left lungs from Balb/c male and female mice were collected at 23 weeks of age for RNA extraction and hybridization on Affymetrix mouse 430 2.0 microarrays. Based on the gender differences and in-utero exposure status, there are 4 groups of mice, females and males, exposed in-utero to filtered-air or SHS. All were exposure to OVA (19-23 weeks). We extracted RNA from 4 animals from each group for microarray analysis (total N = 16 samples).
Experiment type
transcription profiling by array 
Contacts
Rui Xiao <ruixiao85@gmail.com>, Arthur Penn, Paula Polk, Zakia Perveen
Citation
In utero exposure to second-hand smoke aggravates the response to ovalbumin in adult mice. Xiao R, Perveen Z, Rouse RL, Le Donne V, Paulsen DB, Ambalavanan N, Penn AL.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-38409.idf.txt
Sample and data relationshipE-GEOD-38409.sdrf.txt
Raw data (1)E-GEOD-38409.raw.1.zip
Processed data (1)E-GEOD-38409.processed.1.zip
Array designA-AFFY-45.adf.txt
Links