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E-GEOD-38273 - ChIP-seq for SCML2 and SCML2 mutants in 293T-REx and K562 cells

Status
Released on 2 July 2014, last updated on 31 July 2014
Organism
Homo sapiens
Samples (39)
Protocols (7)
Description
Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a mammalian homolog of Drosophila SCM, a Polycomb-group protein that associates with PRC1. Here, we show that SCML2A, an SCML2 isoform tightly associated to chromatin, contributes to PRC1 localization and also directly enforces repression of certain Polycomb target genes. SCML2A binds to PRC1 via its SPM domain and interacts with ncRNAs through a novel RNA-binding region (RBR). Targeting of SCML2A to chromatin involves the coordinated action of the MBT domains, RNA binding, and interaction with PRC1 through the SPM domain. Deletion of the RBR reduces the occupancy of SCML2A at target genes and overexpression of a mutant SCML2A lacking the RBR causes defects in PRC1 recruitment. These observations point to a role for ncRNAs in regulating SCML2 function and suggest that SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1. This is the ChIP-seq part of the study
Experiment type
ChIP-seq 
Contacts
Roberto Bonasio <geo@ncbi.nlm.nih.gov>, Danny Reinberg, Emilio Lecona
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-38273.idf.txt
Sample and data relationshipE-GEOD-38273.sdrf.txt
Processed data (20)Click to browse processed data
Links