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E-GEOD-38261 - Distinct Transcriptional Signatures of Bone Marrow-Derived C57BL/6 and DBA/2 Dendritic Leucocytes Hosting Live Leishmania amazonensis Amastigotes

Status
Released on 7 January 2013, last updated on 14 January 2013
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (8)
Description
To determine the modulation of gene expression of C57BL/6 and DBA/2 BMDLs in the presence of living intracellular Leishmania amazonensis amastigotes A genome-wide transcriptional analysis was performed by comparing the gene expression profiles of control DLs and live amastigote-hosting DLs from both mouse strains. Dendritic Leucocytes were generated in vitro from bone marrow progenitors (C57Bl/6 and DBA/2 mice). Leishmania amazonensis amastigotes were purified from mouse cutaneous lesions and were added to DL cultures. After 24h, and following a sorting procedure, only BMDls housing living amastigotes were selected for total RNA extraction. Three Biological replicates per condition were run.
Experiment type
transcription profiling by array 
Contacts
Emilie de la Llave, Eric Prina, Geneviève Milon, Guillaume Soubigou, Hervé Lecoeur, Jean-Yves Coppée, Thierry Lang
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-38261.idf.txt
Sample and data relationshipE-GEOD-38261.sdrf.txt
Raw data (1)E-GEOD-38261.raw.1.zip
Processed data (1)E-GEOD-38261.processed.1.zip
Additional data (1)E-GEOD-38261.additional.1.zip
Array designA-AFFY-130.adf.txt
Links