ID_REF = VALUE = normalized log10 ratio (Cy5/Cy3) representing test/reference
Agilent Feature Extraction Software (v 220.127.116.11) was used for background subtraction and LOWESS normalization.
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.18.104.22.168).
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
100 ng-input two-round RT/IVT RNA amplification protocol
Adipose tissues (eWAT) were isolated from the db/db and the lean control (db/+) mice at 10-week of age. The db/db mice are obese, severely hyperglycemic and only modestly hyperinsulinemic at 10 weeks of age.
Total RNA extracted using Trizol following manufacturer's instructions