E-GEOD-37918 - RNA-seq in breast cancer cell lines after manipulation of miR-23b expression

Released on 1 May 2013, last updated on 9 May 2013
Homo sapiens
Samples (4)
Protocols (4)
RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-23b in both MCF-7 and MDA-MB-231 breast cancer cell lines Our study implicates miR-23b in cytoskeletal remodeling in breast cancer. To evaluate the entire set of genes modulated by miR-23b we performed RNA-seq after ectopic manipulation of this miRNA in breast cancer cell lines. We over-expressed this miRNA in MCF-7 epithelial cancer cell lines and also reduced its activity by stably transfecting MDA-MB-231 mesenchymal-like cancer cell lines with a specific sponge vector. RNA-seq analysis revealed a number of candidate targets of this miRNA.
Experiment type
RNA-seq of coding RNA 
miR-23b regulates cytoskeletal remodeling, motility and metastasis by directly targeting multiple transcripts. Pellegrino L, Stebbing J, Braga VM, Frampton AE, Jacob J, Buluwela L, Jiao LR, Periyasamy M, Madsen CD, Caley MP, Ottaviani S, Roca-Alonso L, El-Bahrawy M, Coombes RC, Krell J, Castellano L. , Europe PMC 23580553
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-37918.idf.txt
Sample and data relationshipE-GEOD-37918.sdrf.txt
Processed data (1)E-GEOD-37918.processed.1.zip