E-GEOD-37778 - ChIP-chip of Geobacter sulfurreducens PCA with antibody against various sigma factors under various conditions

Status
Released on 1 May 2013, last updated on 2 June 2014
Organism
, Geobacter sulfurreducens PCA
Samples (20)
Array (1)
Protocols (12)
Description
We applied a ChIP-chip approach to elucidate the binding profiles of sigma factors (RpH, RpoN and RpoS) experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 10 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture condtions against three alternative sigma factors . The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).
Experiment type
transcription profiling by tiling array 
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-37778.idf.txt
Sample and data relationshipE-GEOD-37778.sdrf.txt
Raw data (2)E-GEOD-37778.raw.1.zip, E-GEOD-37778.raw.2.zip
Processed data (1)E-GEOD-37778.processed.1.zip
Array designA-GEOD-9112.adf.txt
Links