8 protocols
AccessionType
normalization data transformation protocol
RMA through R-based affy module. ID_REF = VALUE = RMA value
array scanning protocol
Arrays were scanned using a GenePix 4000B microarray scanner.
hybridization protocol
Hybridization was performed according to standard protocols for Affymetrix HG-U133 Plus 2.0 arrays.
labelling protocol
Biotinylated cRNA was generated using standard Affymetrix protocols.
nucleic acid extraction protocol
Total RNA were extracted by the RNeasy mini kit (Cat. 74106; Qiagen, Hilden, Germany), and 100 ng to 1 ug of total RNA were subjected to reverse transcription using the SuperScriptTM III Reverse transcriptase kit (Invitrogen) as directed by the manufacturer.
sample treatment protocol
HT29 cells were transduced with an miR-146a expression construct using a lentivirus system.
growth protocol
The medium for this cell line is 10% FBS-DMEM. atmosphere: air, 95%; carbon dioxide (CO2), 5% temperature: 37.0°C
sample treatment protocol
HT29 cells were transduced with an empty vector using a lentivirus system.