E-GEOD-37250 - Genome-wide transcriptional profiling of HIV positive and negative adults with active tuberculosis, latent TB infection and other diseases

Released on 23 October 2013, last updated on 2 June 2014
Homo sapiens
Samples (537)
Array (1)
Protocols (541)
The study aimed to define transcriptional signatures for detection of active TB (TB) compared to latent TB infection (LTBI) as well as to other diseases (OD) with similar clinical phenotypes in patients with and without HIV in two African adult populations. Transcriptional signatures were identified that distinguished active TB from LTBI, active TB from other diseases, and active TB from both LTBI and other diseases in HIV+/- patients. Adults were recruited from Cape Town, South Africa (n=300) and Karonga, Malawi (n=237) who were either HIV+ or HIV - with either active TB, LTBI or OD. Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human HT-12 Beadchips. Data were analysed in R.
Experiment type
transcription profiling by array 
Victoria Wright <v.wright@imperial.ac.uk>, Amelia C Crampin, Brian Eley, Florian Kern, Hazel M Dockrell, Lachlan J Coin, Ling Ling, Martin L Hibberd, Michael Levin, Myrsini Kaforou, Neil French, Paul R Langford, Robert J Wilkinson, Robert S Heyderman, Suzanne T Anderson, Victoria J Wright