E-GEOD-37049 - Analysis of gene expression in differentiated human primary keratinocytes depleted for Grainyhead like 3 (GRHL3)
Released on 18 July 2012, last updated on 25 September 2015
Human primary keratinocytes were depleted of GRHL3 by siRNA and induced to differentiated for 2 days by addition of Calcium Primary normal human keratinocytes were transfected with GRHL3 or scrambled control siRNA using RNAi max (Life Technologies). 24 hours post transfection medium was raised to 1.8mM to induce differentiation. Cells were collected 48 hours later.
transcription profiling by array
Amelia Soto Hopkin <email@example.com>, Amelia S Hopkin, Bogi Andersen
GRHL3/GET1 and trithorax group members collaborate to activate the epidermal progenitor differentiation program. Hopkin AS, Gordon W, Klein RH, Espitia F, Daily K, Zeller M, Baldi P, Andersen B. , Europe PMC 22829784