E-GEOD-36980 - Microarray analysis of postmortem human brains with presence or absence of Alzheimer's disease

Released on 17 April 2013, last updated on 7 September 2017
Homo sapiens
Samples (79)
Array (1)
Protocols (7)
To identify molecular pathological alterations in AD brains, we performed interspecies comparative microarray analyses using RNAs prepared from postmortem human brain tissues donated for the Hisayama study and hippocampal RNAs from the triple-transgenic mouse model of AD (3xTg-AD) Three-way ANOVA of microarray data from frontal cortex, temporal cortex and hippocampus with presence/absence of AD and vascular dementia, and sex, as factors revealed that the gene expression profile is most significantly altered in the hippocampi of AD brains. Comparative analyses of the brains of AD patients and a mouse model of AD showed that genes involved in non-insulin dependent DM and obesity were significantly altered in both, as were genes related to psychiatric disorders and Alzheimer’s disease. We prepared RNA samples from the gray matter of frontal and temporal cortices and hippocampi derived from 88 postmortem brains, among which 26 cases were pathologically diagnosed as having AD or an AD-like disorder. High-quality RNA (RIN≧6.9) samples were subjected to microarray analysis using the Affymetrix Human Gene 1.0 ST platform, and only those results that passed examinations for quality assurance and quality control of the Human Gene 1.0 ST arrays were retrieved. In total, we obtained gene expression profiles from the following samples: 33 frontal cortex samples, among which 15 were from AD patients; 29 temporal cortex samples, among which 10 were from AD patients; 17 hippocampus samples, among which seven were from AD patients
Experiment type
transcription profiling by array 
Altered expression of diabetes-related genes in Alzheimer's disease brains: the Hisayama study. Hokama M, Oka S, Leon J, Ninomiya T, Honda H, Sasaki K, Iwaki T, Ohara T, Sasaki T, LaFerla FM, Kiyohara Y, Nakabeppu Y. , Europe PMC 23595620
Investigation descriptionE-GEOD-36980.idf.txt
Sample and data relationshipE-GEOD-36980.sdrf.txt
Raw data (2)E-GEOD-36980.raw.1.zip, E-GEOD-36980.raw.2.zip
Processed data (1)E-GEOD-36980.processed.1.zip
Array designA-AFFY-141.adf.txt