Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-36947 - Elevating Sox2 levels deleteriously affects the growth of glioblastoma and medulloblastoma cells

Status
Released on 25 August 2012, last updated on 26 September 2012
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (8)
Description
Induction of the transcription factor Sox2 from a doxycycline-inducible promoter in iSox2-DAOY medulloblastoma cells. Affymetrix microarrays were used to characterize the gene expression profile of iSox2-DAOY cells in the absence and presence of doxycycline. Human DAOY medulloblastoma cells were engineered to express epitope tagged Sox2 under the control of a doxycycline inducible promoter, to produce iSox2-DAOY cells. iSox2-DAOY cells were FACS sorted into CD133 low and high populations. The CD133 low population was cultured in the absence and presence of 0.5 µg/mL doxycycline for 24 hours. RNA was extracted from cells cultured without and with doxycycline, and was used for microarray analysis.
Experiment type
transcription profiling by array 
Contacts
Angie Rizzinio <arizzino@unmc.edu>, Angie Rizzino, Jesse L Cox, Phillip J Wilder
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-36947.idf.txt
Sample and data relationshipE-GEOD-36947.sdrf.txt
Raw data (1)E-GEOD-36947.raw.1.zip
Processed data (1)E-GEOD-36947.processed.1.zip
Array designA-AFFY-141.adf.txt
R ExpressionSetE-GEOD-36947.eSet.r
Links