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E-GEOD-36926 - The Epstein-Barr virus induced tumor suppressor miR-34a is growth promoting in EBV-infected B cells

Status
Released on 19 April 2012, last updated on 30 April 2012
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (8)
Description
Epstein-Barr virus (EBV) infection of primary human B cells drives their indefinite proliferation into lymphoblastoid cell lines (LCLs). B cell immortalization depends on expression of viral latency genes as well as the regulation of host genes. Given the important role of miRNAs in regulating fundamental cellular processes, in this study we assayed changes in host miRNA expression during primary B cell infection by EBV. We observed and validated dynamic changes in several miRNAs from early proliferation through immortalization; oncogenic miRNAs were induced and tumor suppressor miRNAs were largely repressed. However, one miRNA described as a p53-targeted tumor suppressor, miR-34a, was strongly induced by EBV infection and expressed in many EBV and KSHV-infected lymphoma cell lines. The EBV latent membrane protein 1 (LMP1) was sufficient to induce miR-34a requiring downstream NFκB activation, but independent of functional p53. Furthermore, over-expression of miR-34a was not toxic in several B lymphoma cell lines and inhibition of miR-34a impaired the growth of EBV transformed cells. This study identifies a pro-growth role for a tumor suppressive miRNA in oncogenic virus-mediated transformation highlighting the importance of studying miRNA function in different cellular contexts. miRNA expression profiling of human B-cells, EBV-infected, proliferating B cells and Monoclonal LCLs from 3 different donors was conducted with the use of up to 2 μg total RNA for sample
Experiment type
transcription profiling by array 
Contacts
Dereje D. Jima <dereje.jima@duke.edu>, Cassandra Jacobs, Christina Chang, Dereje Jima, Eleonora Forte, Eva Gottwein, Micah A Luftig, Qi-Jing Li, Raul Salinas, Sandeep S Dave, Sarah D Linnstaedt, Ting Zhou
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-36926.idf.txt
Sample and data relationshipE-GEOD-36926.sdrf.txt
Raw data (1)E-GEOD-36926.raw.1.zip
Processed data (1)E-GEOD-36926.processed.1.zip
Array designA-MEXP-1469.adf.txt
R ExpressionSetE-GEOD-36926.eSet.r
Links