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E-GEOD-36821 - A novel five-gene signature predicts overall and recurrence-free survival in NSCLC

Status
Released on 30 June 2012, last updated on 17 July 2012
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (7)
Description
Earlier studies had shown that side population cells isolated from established non-small cell lung cancer (NSCLC) cell lines exhibit cancer stem cell properties. Microarray data from side population (SP) and main population (MP) cells isolated from 4 NSCLC lines (A549, H1650, H460, H1975) were used to examine gene expression profiles associated with stemness. Total RNA extracted from SP and MP samples were used to generate cRNA targets, which were hybridized to Human Genome U133 Plus 2.0 probe arrays. Raw data was processed and the mean center expression level for each gene was determined. Four cell lines (A549, H1650, H460, H1975), each having 1 SP and 1 MP sample.
Experiment type
transcription profiling by array 
Contacts
Deepak Perumal, Gregory C Bloom, Sandeep Singh, Sean J Yoder, Srikumar P Chellappan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-36821.idf.txt
Sample and data relationshipE-GEOD-36821.sdrf.txt
Raw data (1)E-GEOD-36821.raw.1.zip
Processed data (1)E-GEOD-36821.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-36821.eSet.r
Links