Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-36379 - Expression data from mouse pancreatic cell lines treated with chromatin-targeting small molecules
Released on 9 March 2012, last updated on 19 March 2012
We measured the genome-wide expression changes induced by 29 compounds targeting HDACs, DNMTs, histone lysine methyltransferases (HKMTs), and protein arginine methyltransferases (PRMTs) in pancreatic α- and β-cell lines. We used microarrays to detail the global programme of gene-expression changes induced by chromatin-targeting compounds at 1, 6, and 24 hours. To identify a comprehensive list of target genes that can be regulated by modulating the activities of chromatin-modifying enzymes, we measured the genome-wide transcriptional effects of 29 compounds in pancreatic alpha and beta cell lines at three time points. The results indicate that compounds cause similar effects independent of the cell line in which they were profiled. All clinical HDAC inhibitors fell into the structural classes of hydroxamic acids and ortho-amino anilides, respectively and up- and down-regulated hundreds of transcripts. In contrast, more selective compounds like the HKMT inhibitor BIX-01294 have specific effects.
transcription profiling by array
Alexander D Gitlin, Alykhan F Shamji, Bradley Taylor, Bridget K Wagner, Dina Fomina-Yadlin, Florence F Wagner, Joshua C Gilbert, Paul A Clemons, Stefan Kubicek, Stuart L Schreiber, Supriya Gupta, Timothy A Lewis, Tuoping Luo, Yuan Yuan