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E-GEOD-36374 - Histone Phosphoacetylation ChIP-seq of Kc167 cells from Drosophila

Status
Released on 29 March 2012, last updated on 3 May 2014
Organism
Drosophila melanogaster
Samples (6)
Protocols (3)
Description
ChIP-seq was performed using Drosophila Kc167 cells using antibodies against H3K4me3 to identify active promoters and H3K4me1 to identify active enhancers. H3K27ac ChIPseq was performed to identify active promoters and enhancers. Once enhancers and promoters were identified, JIL-1 and histone phosphorylation, H3K9acS10ph and H3K27acS28ph, ChIP-seq was performed to look at binding trends. JIL-1 and phosphoacetlation is found at low levels at inactive enhancers and shows increase at active enhancers and promoters. Here we examine histone phosphorylation by JIL-1 and acetylation of H3K27ac by CBP at transcriptionally active vs. inactive promoters and enhancers. ChIP-seq is performed in Kc167 Drosophila cells using antibodies against JIL-1, H3K27acS28ph, H3K9acS10ph, H3K4me3, H3K4me1, and H3K27ac.
Experiment type
ChIP-seq 
Contacts
Edward Ramos, Kevin Van Bortle, Naomi Takenaka, Victor G Corces, Wendy A Kellner
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-36374.idf.txt
Sample and data relationshipE-GEOD-36374.sdrf.txt
Processed data (2)E-GEOD-36374.processed.1.zip, E-GEOD-36374.processed.2.zip
Links