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E-GEOD-36271 - Transcription profiling by array of rice dwarf d10-1 and d14-1 mutants treated with GR24, a synthetic strigolactone analog

Status
Released on 31 December 2013, last updated on 23 November 2015
Organism
Oryza sativa Japonica Group
Samples (18)
Array (1)
Protocols (7)
Description
Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. The phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24. Gene expression profiles in 4-day-old mesocotyls of dwarf (d) mutants (d10-1 and d14-1) and wild-type germinated under dark conditions with or without treatment of 1 μM GR24 were analyzed. Three biological replicates were prepared for each conditions, and a total of eighteen samples were analyzed.
Experiment type
transcription profiling by array 
Contacts
Takaki Yamauchi <geo@ncbi.nlm.nih.gov>, Hiroaki Ichikawa, Itsuro Takamure, Jinghua Yang, Junko Kyozuka, Mikio Nakazono, Nobuhiro Tsutsumi, Shinjiro Yamaguchi, Tomoko Tsuchida-Mayama, Yoshiaki Nagamura, Yusuke Jikumaru, Zhongyuan Hu
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-36271.idf.txt
Sample and data relationshipE-GEOD-36271.sdrf.txt
Raw data (1)E-GEOD-36271.raw.1.zip
Processed data (1)E-GEOD-36271.processed.1.zip
Array designA-GEOD-6864.adf.txt
Links