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E-GEOD-35889 - Expression data from adult wild-type and zCdx1b transgenic zebrafish (3 months old)

Status
Released on 18 February 2012, last updated on 8 March 2012
Organism
Danio rerio
Samples (5)
Array (1)
Protocols (8)
Description
Cdx2 has been suggested to play an important role in Barrett's esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, in vivo data have been lacking. The aim of the present study was to investigate whether transgenic overexpression of zCdx1b, the functional equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish A transgenic zebrafish system was developed by expressing zCdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). zCdx1b expression in the esophageal squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. The upper digestive tract tissue from 3 adult zebrafish (3 months old) was pooled as one sample. Three zCdx1b transgenic samples were used for microarray, and 2 wild type samples were used for control.
Experiment type
transcription profiling by array 
Contacts
Hao Chen <chenh@nccu.edu>, Bo Hu, Xiaoxin Chen
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35889.idf.txt
Sample and data relationshipE-GEOD-35889.sdrf.txt
Raw data (1)E-GEOD-35889.raw.1.zip
Processed data (1)E-GEOD-35889.processed.1.zip
Array designA-AFFY-38.adf.txt
Links