Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-35771 - Real-time quantitative PCR analysis of the expression of neurotransmitter receptor and regulator genes in 2 week old male MeCP2 A140V mutant mouse brain cortex
Released on 1 February 2015, last updated on 8 February 2015
The goal of this study was to identify brain cortex gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A) from SABiosciences. Mice used in this study were 2 week old males. Total RNA was extracted from somatosensory/motor cortex of 2 week old male MeCP2 A140V hemizygous mutant mice and their wild type littermates. For this study, cortical tissue from 3 mice of each genotype was used and RNA from the cortex of each mouse was analyzed separately (ie., triplicate biological replicates). The RNA was purified to remove genomic DNA and was then reverse transcribed for use in quantitative RT-PCR analysis of gene expression using the SABiosciences RT2 profiler PCR array system. The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A).
transcription profiling by RT-PCR
Garilyn Jentarra <firstname.lastname@example.org>, Garilyn M Jentarra, Vinodh Narayanan