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E-GEOD-35685 - Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin

Status
Released on 14 August 2012, last updated on 20 August 2012
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (8)
Description
Studies of adult human hematopoiesis have until now relied on the expression of CD10 to define lymphoid commitment. We report a novel lymphoid-primed population in human bone marrow that is generated from hematopoietic stem cells (HSC) prior to the onset of CD10 expression and B cell commitment, and is identified by high levels of the homing molecule L-selectin (CD62L). CD10-CD62Lhi progenitors have full lymphoid (B/T/NK) potential, and show reduced myeloid and absent erythroid potential. Genome-wide gene expression analysis demonstrates that the CD10-CD62Lhi population represents an intermediate stage of differentiation between CD34+CD38- HSC and CD34+lin-CD10+ progenitors marked by down-regulation of TAL1 and MPL, upregulation of E2A, CD3E and IL2RG expression, and absent B cell commitment or RAG1/2 expression. Immature CD34+CD1a- thymocytes are also CD62Lhi and L-selectin ligands are expressed at the cortico-medullary junction, suggesting a possible role for L-selectin in human thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow. Freshly harvested human bone marrow was ficoll purified, enriched for CD34+, and then FACS sorted. It was then sorted into 3 samples CD34+CD38-, CD34+ CD10- CD62L++, and CD34+ CD10+, with 3 independent biological replicates
Experiment type
transcription profiling by array 
Contacts
Lisa Kohn <Lakohn@ucla.edu>, Chintan Parekh, Gay M Crooks, Hanna Mikkola, Judy Zhu, Lisa A Kohn, Qian-Lin Hao, Rajkumar Sasidharan, Shundi Ge
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35685.idf.txt
Sample and data relationshipE-GEOD-35685.sdrf.txt
Raw data (1)E-GEOD-35685.raw.1.zip
Processed data (1)E-GEOD-35685.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-35685.eSet.r
Links