E-GEOD-3566 - Transcription profiling by array of Drosophila Kc167 cells with RNAi knockdown of Enigma, a mitochondrial protein with homology to acyl-CoA dehydrogenases
Released on 31 July 2007, last updated on 30 April 2015
5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Experiment Overall Design: Kc-167 cells, 5 day treatment with Enigma-specific RNAi or beta-lactamase control RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot for Enigma.
transcription profiling by array, cellular modification
Enigma, a mitochondrial protein affecting lifespan and oxidative stress response in Drosophila. Philippos Mourikis,Gregory D Hurlbut,Spyros Artavanis-Tsakonas.