E-GEOD-3558 - Effect of in vitro transcription time on the fidelity of T7-based RNA linear amplification
Submitted on 3 November 2005, released on 3 November 2005, last updated on 18 October 2011
Zhao et al. Amplification Figure 1 This experiment was designed to evaluate the effect of in vitro transcription time on the fidelity, reproducibility, and yield of T7 based linear amplification. Duplicate reactions were performed at 37 degree for 2, 3, 4, 5, and 6 hours. Two additional 5-hour incubation reactions were stored at 4 degree overnight to determine the effect of low temperature incubation on amplification. BC2 total RNA was amplified using the Jeffrey lab protocol with the G50 column cleanup step. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
unknown experiment type
Stanford Microarray Database <firstname.lastname@example.org>, Hongjuan Zhao
Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis. Zhao H, Hastie T, Whitfield ML, Børresen-Dale AL, Jeffrey SS.