E-GEOD-3557 - Effect of the amount of input total RNA on T7 amplification
Submitted on 3 November 2005, released on 3 November 2005, last updated on 18 October 2011
Zhao et al. Amplification Table 9 This experiment was designed to evaluate the effect of the amount of input total RNA on the fidelity, reproducibility, and yield of T7 based RNA linear amplification. BC2 total RNA was amplified using the Jeffrey lab protocol. Different amounts of T7 primer were used according to the quantity of input total RNA. Multiple amplifications were done for each quantity of input total RNA to minimize experimental variations. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
unknown experiment type
Stanford Microarray Database <firstname.lastname@example.org>, Hongjuan Zhao
Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis. Zhao H, Hastie T, Whitfield ML, Børresen-Dale AL, Jeffrey SS.