E-GEOD-3557 - Effect of the amount of input total RNA on T7 amplification

Status
Submitted on 3 November 2005, released on 3 November 2005, last updated on 18 October 2011
Organism
Homo sapiens
Samples (18)
Arrays (2)
Protocols (2)
Description
Zhao et al. Amplification Table 9 This experiment was designed to evaluate the effect of the amount of input total RNA on the fidelity, reproducibility, and yield of T7 based RNA linear amplification. BC2 total RNA was amplified using the Jeffrey lab protocol. Different amounts of T7 primer were used according to the quantity of input total RNA. Multiple amplifications were done for each quantity of input total RNA to minimize experimental variations. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
Experiment type
unknown experiment type 
Contacts
Citation
Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis. Zhao H, Hastie T, Whitfield ML, Børresen-Dale AL, Jeffrey SS.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-3557.idf.txt
Sample and data relationshipE-GEOD-3557.sdrf.txt
Processed data (1)E-GEOD-3557.processed.1.zip
Array designsA-GEOD-1282.adf.txt, A-GEOD-3047.adf.txt
Links