E-GEOD-35507 - CSB3 transcriptome analysis (Arabidopsis thaliana)

Status
Released on 25 April 2014, last updated on 2 June 2014
Organism
Arabidopsis thaliana
Samples (2)
Array (1)
Protocols (6)
Description
In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens. 5 five-week-old plants were collected, frozen in liquid nitrogen and stored at -80ºC. Samples from Col-0 and csb3 were compared.
Experiment type
transcription profiling by array 
Contacts
Pablo Vera <vera@ibmcp.upv.es>, Ana López, María J Gil, Vicente Ramírez
Citation
An extracellular subtilase switch for immune priming in Arabidopsis. Ram�rez V, L�pez A, Mauch-Mani B, Gil MJ, Vera P. , Europe PMC 23818851
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35507.idf.txt
Sample and data relationshipE-GEOD-35507.sdrf.txt
Processed data (1)E-GEOD-35507.processed.1.zip
Array designA-AFFY-2.adf.txt
Links