E-GEOD-35232 - Transcription profile of adipose derived mesenchymal stem cells separated by cell surface protein N-cadherin

Status
Released on 29 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (3)
Array (1)
Protocols (7)
Description
Mesenchymal stem cells (MSCs) are one of most promising stem cell sources for regenerative medicine. MSCs have a differention ability into several tissues. However, their differentiation efficiency is considered quite limited. Especially, the differentiation efficiency into cardiomyocytes is very low. N-cadherin is one of the cell surface protein that is expressed in developing and adult cardiomyocytes. We found that cell surface expression of N-cadherin in MSCs shows good correlation with the cardiomyogenic differentiation ability. We also analyzed the expression profiles of N-cadherin-positive and N-cadherin-negative fraction by microarray. We found that N-cadherin-positive MSCs show higher expression of some of the cardiomyogenesis-related genes than N-cadherin-negative MSCs. The ASCs were maintained in MesenPRO RSTM Medium. The ASCs were harvested in a cell dissociation buffer. The ASCs were incubated with biotinylated anti N-cadherin antibody and next incubated with streptavidin –APC. Subsequently cells were incubated with anti-APC microbeads. Immunomagnetic separation of N-cadherin+ cells were performed with autoMACSTMPro separator applying the separation program ‘depl025’
Experiment type
transcription profiling by array 
Contacts
Hisako Ishimine <geo@ncbi.nlm.nih.gov>, Akira Kurisaki, Makoto Asashima
Citation
N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation. Ishimine H, Yamakawa N, Sasao M, Tadokoro M, Kami D, Komazaki S, Tokuhara M, Takada H, Ito Y, Kuno S, Yoshimura K, Umezawa A, Ohgushi H, Asashima M, Kurisaki A. , Europe PMC 23899519
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35232.idf.txt
Sample and data relationshipE-GEOD-35232.sdrf.txt
Raw data (1)E-GEOD-35232.raw.1.zip
Processed data (1)E-GEOD-35232.processed.1.zip
Array designA-AGIL-28.adf.txt
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