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E-GEOD-35187 - Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72

Status
Released on 30 November 2012, last updated on 4 May 2014
Organism
Azoarcus sp.
Samples (12)
Array (1)
Protocols (8)
Description
Endophytic colonization is a very complex process which is not yet completely understood. Molecules exuded by the plants may act as signals which influence the ability of the microbe to colonize the host or survive in the rhizosphere. Here we investigated whether root exudates of the host might play a role in initiating the endophyte-rice interaction. The whole genome microarray approach was used to investigate the response of the diazotrophic model endophyte, Azoarcus sp. strain BH72, to exudates of O. sativa cv. Nipponbare in order to identify differentially regulated genes. Azoarcus sp. strain BH72 was grown in the presence or absence of root exudates of Oryza sativa cv. Nipponbare for two different time points, and differences in the gene expression profile were monitored. RNA from cells grown on synthetic medium for 1 and 4 hours respectively in presence (experiment) and absence (control) of exudates was used for two color whole genome microarray approach.
Experiment type
transcription profiling by array 
Contacts
Barbara Reinhold-Hurek <breinhold@uni-bremen.de>, A Becker, B Reinhold-Hurek, T Shidore
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35187.idf.txt
Sample and data relationshipE-GEOD-35187.sdrf.txt
Raw data (1)E-GEOD-35187.raw.1.zip
Processed data (1)E-GEOD-35187.processed.1.zip
Array designA-GEOD-11300.adf.txt
Links