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E-GEOD-35174 - Development of miRNA expression signatures of MEFs deficient in a ER stress mediator XBP1 for tunicamycin treatment

Status
Released on 18 January 2015, last updated on 28 January 2015
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted. Among them, expression of three miRNAs (miR-23a, miR-27a, miR-24-2) was quantified in the RNA samples from the same as the microarray, and COS7 cells by real-time PCR, confirming existence of similar mechanisms of trancriptional repression in ER stress by tunicamycin treatment. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with 2ug/mL tunicamycin for 12 or 24 hrs. Two independent experiments were performed at each time (untreated, 12 or 24 hrs). miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted.
Experiment type
transcription profiling by array 
Contacts
Chinobu Miyamoto, Kazue Tsugawa, Kazuna Takahara, Kiyoe Kurahashi, Mari Funahashi, Masato Miyake, Miho Oyadomari, Naoko Miura, Robert Zheng, Ryosuke Sato, Seiichi Oyadomari, Taiji Ito, Tomoko Mori
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35174.idf.txt
Sample and data relationshipE-GEOD-35174.sdrf.txt
Raw data (1)E-GEOD-35174.raw.1.zip
Processed data (1)E-GEOD-35174.processed.1.zip
Array designA-GEOD-13493.adf.txt
Links