Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-35174 - Development of miRNA expression signatures of MEFs deficient in a ER stress mediator XBP1 for tunicamycin treatment
Released on 18 January 2015, last updated on 28 January 2015
To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted. Among them, expression of three miRNAs (miR-23a, miR-27a, miR-24-2) was quantified in the RNA samples from the same as the microarray, and COS7 cells by real-time PCR, confirming existence of similar mechanisms of trancriptional repression in ER stress by tunicamycin treatment. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with 2ug/mL tunicamycin for 12 or 24 hrs. Two independent experiments were performed at each time (untreated, 12 or 24 hrs). miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted.
transcription profiling by array
Chinobu Miyamoto, Kazue Tsugawa, Kazuna Takahara, Kiyoe Kurahashi, Mari Funahashi, Masato Miyake, Miho Oyadomari, Naoko Miura, Robert Zheng, Ryosuke Sato, Seiichi Oyadomari, Taiji Ito, Tomoko Mori