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E-GEOD-35139 - Congenic dissection of a major QTL for methamphetamine sensitivity implicates epistasis

Status
Released on 18 January 2012, last updated on 23 January 2012
Organism
Mus musculus
Samples (28)
Array (1)
Protocols (7)
Description
We identified a congenic mouse with an introgressed region from the A/J donor inbred strain on an inbred C57BL/6J background that showed a reduced locomotor stimulant response to methamphetamine. We conducted microarray analysis of the striatum from drug-naive female and male mice that were 6-9 weeks old. The congenic region is on chromosome 11 and spans approximately 84-96 Mb. There were two groups of mice used in the analysis: B6 control mice versus congenic mice. Congenic mice were collapsed across heterozygous and homozygous genotypes. All mice are on a C57BL/6J background. Congenic mice differ only on chromosome 11 where they contain the introgressed A/J alleles from 84-96 Mb. 14 C57BL/6J wildtype mice and 14 B6.A/J congenic mice (84-96 Mb region from A/J strain) were examined for gene expression differences using striatal tissue (left and right punches pooled). All mice were drug-naïve and were 6-9 weeks old at the time of sacrifice.
Experiment type
transcription profiling by array 
Contacts
Camron D Bryant <camron@uchicago.edu>, Abraham Palmer, Greta Sokoloff, Loren A Kole, Michael A Guido
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35139.idf.txt
Sample and data relationshipE-GEOD-35139.sdrf.txt
Raw data (1)E-GEOD-35139.raw.1.zip
Processed data (1)E-GEOD-35139.processed.1.zip
Array designA-AFFY-130.adf.txt
R ExpressionSetE-GEOD-35139.eSet.r
Links