8 protocols
AccessionType
bioassay_data_transformation
ID_REF =
VALUE = normalized, log2-transformed signal
labeling
As suggested by Agilent
nucleic_acid_extraction
total RNA was purified with Rneasy kit (Qiagen) following the manifacturers protocol
image_aquisition
As suggested by Agilent using the illumina scanner
grow
Cells where isolated from DMD/MDX mice using FACS (Fluorescence Activade Cell Sort); Sca1+ muscle interstitial cells were isolated as CD34+/a7-integrin+/Sca1+ while MuSC were isolated as CD34+/a7-integrin+/Sca1-
specified_biomaterial_action
Mice were treated with TSA (0.6 mg/Kg) or veichle for 45 days (i.p. injection)
hybridization
As recommended by Agilent
feature_extraction
Raw data filtered on the absent / detected flag calculated by the Agilent algorithum in GeneSpring GX11 (The data was normalized with quantiles).