E-GEOD-34834 - Role and function of SPRY1/2 in BCR-ABL1 driven pre-B cells
Released on 3 June 2013, last updated on 17 June 2013
In order to investigate the function of SPRY1 and SPRY2 in ALL, we isolated bone marrow cells from Spry1 and 2 fl/fl mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with CRE-ERT2 or ERT2 (PMIP) and after 2 days of treatment with 4OH-Tamoxifen (0.5 micromolar) subjected to gene expression analysis. Two days after treatment with 4OH-Tamoxifen (0.5 micromolar) total RNA of Puromycin resistant cells was extracted and subjected to gene expression analysis.
transcription profiling by array
Markus Müschen <firstname.lastname@example.org>, seyedmehdi shojaee