E-GEOD-34834 - Role and function of SPRY1/2 in BCR-ABL1 driven pre-B cells

Status
Released on 3 June 2013, last updated on 17 June 2013
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (5)
Description
In order to investigate the function of SPRY1 and SPRY2 in ALL, we isolated bone marrow cells from Spry1 and 2 fl/fl mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with CRE-ERT2 or ERT2 (PMIP) and after 2 days of treatment with 4OH-Tamoxifen (0.5 micromolar) subjected to gene expression analysis. Two days after treatment with 4OH-Tamoxifen (0.5 micromolar) total RNA of Puromycin resistant cells was extracted and subjected to gene expression analysis.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-34834.idf.txt
Sample and data relationshipE-GEOD-34834.sdrf.txt
Raw data (1)E-GEOD-34834.raw.1.zip
Processed data (1)E-GEOD-34834.processed.1.zip
Array designA-AFFY-130.adf.txt
Links