E-GEOD-34586 - Comparison of the transcripts in control and Blimp-1-deficient keratinocytes

Released on 2 April 2013, last updated on 8 April 2013
Mus musculus
Samples (4)
Array (1)
Protocols (8)
We performed microarray analysis to examine the differential gene expression profiles between Prdm1 (Blimp-1)-deleted and control keratinocytes. Keratinocytes isolated from Prdm1-floxed K5-CreER positive (CKO) mice were cultured in the presence of 4OHT to induce deletion of the Prdm1 allele in vitro. Prdm1-floxed K5-CreER positive (CKO) keratinocytes treated with the ethanol solvent control (EtOH) or Prdm1-floxed K5-CreER negative (control) keratinocytes treated with 4OHT or EtOH served as controls. Microarray analyses revealed that there were 93 genes up-regulated and 109 genes down-regulated by more than 2-fold in the CKO + 4OHT group in comparison with the CKO + EtOH, Ctrl + 4OHT or Ctrl + EtOH groups. Several corneocytes-related genes, including Rptn, Lce1f, Krt1 and Lce1d, are significantly down-regulated and several cytokines/chemokines, including Cxcl1, Cxcl2, Cxcl5 and Il24, are significantly up-regulated upon the deletion of Prdm1 in vitro. We analyzed the transcription profiles in control and Blimp-1-deficient keratinocytes using the GeneChip® Mouse Expression Array 430A.
Experiment type
transcription profiling by array 
Ming-Feng Chiang <geo@ncbi.nlm.nih.gov>, I Y Lin, K I Lin, Ming F Chiang
Investigation descriptionE-GEOD-34586.idf.txt
Sample and data relationshipE-GEOD-34586.sdrf.txt
Raw data (1)E-GEOD-34586.raw.1.zip
Processed data (1)E-GEOD-34586.processed.1.zip
Array designA-AFFY-23.adf.txt