15 protocols
AccessionType
feature_extraction
elav_10to12h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
toll10b_02to04h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
grow
D. melanogaster embryos were collected and aged on yeasted apple juice plates at 25 °C and 60 % humidity.
feature_extraction
mef2_06to08h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
nucleic acid library construction protocol
mRNA-seq: polyA mRNA was purified from total RNA in two consecutive rounds of oligo(dT) selection. After RNA fragmentation and reverse transcription sequencing libraries were prepared following Illumina's instructions (see ChIP-seq)
feature_extraction
mef2_14to17h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
mef2_14to17h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
mef2_10to12h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
mef2_10to12h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
mef2_08to10h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
mef2_08to10h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
mef2_06to08h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
elav_14to17h_2.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
toll10b_02to04h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file
feature_extraction
elav_14to17h_1.bam; genome build: dm3 Reads were aligned to the UCSC dm3 genome using Tophat with a FlyBase release 5.28 GFF annotation file