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E-GEOD-34281 - Poised RNA Polymerase II changes over developmental time and prepares genes for future expression (RNA-seq data)

Status
Released on 20 December 2012, last updated on 14 January 2013
Organism
Drosophila melanogaster
Samples (13)
Protocols (15)
Description
Poised RNA polymerase II is predominantly found at developmental control genes and is thought to allow their rapid and synchronous induction in response to extracellular signals. How the recruitment of poised RNA Pol II is regulated during development is not known. By isolating muscle tissue from Drosophila embryos at five stages of differentiation, we show that the recruitment of poised Pol II occurs at many genes de novo and this makes them permissive for future gene expression. When compared to other tissues, these changes are stage-specific and not tissue-specific. In contrast, Polycomb group repression is tissue-specific and in combination with Pol II (the balanced state) marks genes with highly dynamic expression. This suggests that poised Pol II is temporally regulated and is held in check in a tissue-specific fashion. We compare our data to mammalian embryonic stem cells and discuss a framework for predicting developmental programs based on chromatin state. mRNA-seq of Drosophila tissues during development
Experiment type
RNA-seq of coding RNA 
Contacts
Jeff Johnston <jjj@stowers.org>, Ariel Paulson, Bjoern Gaertner, Julia Zeitlinger, Kai Chen, Karin Zueckert-Gaudenz
Citation
Poised RNA Polymerase II Changes over Developmental Time and Prepares Genes for Future Expression. Gaertner B, Johnston J, Chen K, Wallaschek N, Paulson A, Garruss AS, Gaudenz K, De Kumar B, Krumlauf R, Zeitlinger J. , PMID:23260668
MINSEQE
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