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E-GEOD-3303 - Transcription profiling by array of adult zebrafish control and post-lesion retina to investigate the molecular determinants of retinal regeneration in adult vertebrate

Status
Released on 16 September 2005, last updated on 14 December 2012
Organism
Danio rerio
Samples (12)
Array (1)
Protocols (13)
Description
Purpose: Investigate the molecular determinants of retinal regeneration in adult vertebrates by analyzing the gene expression profiles of control and post-lesion retina of adult zebrafish, a system that regenerates following injury. Methods: Gene expression profiles of zebrafish retina and brain were determined with DNA microarray, RT-PCR, and real-time quantitative PCR analyses. Damaged retinas and their corresponding controls were analyzed 2-5 days post-lesion (acute injury condition) or 14 d post-lesion (cell regeneration condition). Results: Expected similarities and differences in the gene expression profile of zebrafish retina and brain were observed, confirming the applicability of the gene expression techniques. Mechanical lesion of retina triggered significant, time-dependent changes in retinal gene expression. The induced transcriptional changes were consistent with cellular phenomena known to occur, in a time-dependent manner, subsequent to retinal lesion, including cell cycle progression, axonal regeneration, and regenerative cytogenesis. Conclusions: The results indicate that retinal regeneration in adult zebrafish involves a complex set of induced, targeted changes in gene transcription, and suggest that these molecular changes underlie the ability of the adult vertebrate retina to regenerate. Keywords: time course; injury response; cellular correlation Control brain and retina (unlesioned); Control and lesioned retina (matched animals, at least n = 8 for each condition).
Experiment type
transcription profiling by array 
Contacts
David A Cameron <camerond@upstate.edu>, Frank A Middleton, Karen L Gentile, Patrick Yurco
Citation
Gene expression profiles of intact and regenerating zebrafish retina. Cameron DA, Gentile KL, Middleton FA, Yurco P.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-3303.idf.txt
Sample and data relationshipE-GEOD-3303.sdrf.txt
Processed data (1)E-GEOD-3303.processed.1.zip
Array designA-AFFY-38.adf.txt
Links