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E-GEOD-32998 - Gene Array Analyzer (GAA): Alternative usage of gene arrays to study alternative splicing events (MoEx array)

Status
Released on 28 November 2011, last updated on 25 June 2012
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
The latest version of microarrays released by Affymetrix, the GeneChip Gene 1.0 ST Arrays (gene arrays), are designed in a similar fashion as exon arrays, which enables to identify differentially expressed exons, rather than only the expression level of whole transcripts. Here, we propose an extension, Gene Array Analyzer (GAA), to our previously published Exon Array Analyzer (EAA). GAA enables to analyse gene arrays on exon level and therefore supports to identify alternative splicing with gene arrays. To show the applicability of GAA, we used gene arrays to profile alternative splice events during the development of the heart. Further re-analysis of published gene arrays could show, that some of these splice events reoccur under pathological conditions. The web interface of GAA is user friendly, functional without set up and freely available at http://GAA.mpi-bn.mpg.de. Alternative splicing and gene expression analysis during development of the heart and cardiomyoyte differentiation.
Experiment type
transcription profiling by array 
Contacts
Pascal Gellert <pascal.gellert@mpi-bn.mpg.de>, David John, Karsten grosse Kreymborg, Katharina Jenniches, Mizue Teranishi, Piera De Gaspari, Shizuka Uchida, Thomas Braun
Citation
Gene Array Analyzer: alternative usage of gene arrays to study alternative splicing events. Gellert P, Teranishi M, Jenniches K, De Gaspari P, John D, Kreymborg Kg, Braun T, Uchida S. , PMID:22123740
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32998.idf.txt
Sample and data relationshipE-GEOD-32998.sdrf.txt
Raw data (2)E-GEOD-32998.raw.1.zip, E-GEOD-32998.raw.2.zip
Processed data (1)E-GEOD-32998.processed.1.zip
Array designA-AFFY-98.adf.txt
Links