Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-32923 - The NIH Human Pluripotent Stem Cell Database (Agilent, mRNA)

Status
Released on 31 October 2012, last updated on 8 November 2012
Organism
Homo sapiens
Samples (230)
Array (1)
Protocols (8)
Description
To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov. 230 samples: 44 human ESC (UNDIFF), 16 human iPSC (UNDIFF) 44 human ESC (KSR), 15 human iPSC (KSR), 44 human ESC (FBS), 15 human iPSC (FBS), 1 human Brain, 1 human Placenta, 1 human Skeletal Muscle, 1 human Heart, 1 human Liver, 1 human Lung, 1 human Kidney, 1 human Esophagus, 1 human Ovary, 1 human Testes, 1 human Spleen, 1 human Adipose, 1 human Thyroid, 1 human Thymus, 1 human Small Intestine, 1 human Prostate, 1 human Cervix, 1 human Colon, 1 human Bladder, 1 human Trachea) and 32 processing controls (UniRef).
Experiment type
transcription profiling by array 
Contacts
Kory R Johnson <johnsonko@ninds.nih.gov>, Amarendra S Yavatkar, Barbara S Mallon, Josh G Chenoweth, Kevin G Chen, Kye Y Park, Leonard J Tyson, Paul J Tesar, Rebecca S Hamilton, Ronald D McKay, Yang C Fann
Citation
StemCellDB: The Human Pluripotent Stem Cell Database at the National Institutes of Health. Mallon BS, Chenoweth JG, Johnson KR, Hamilton RS, Tesar PJ, Yavatkar AS, Tyson LJ, Park K, Chen KG, Fann YC, McKay RD. , PMID:23117585
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