Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-32733 - H3K18Ac (new lot).D.mel 3rd Instar Larvae Nuclei

Status
Released on 12 October 2011, last updated on 2 March 2012
Organism
Drosophila melanogaster
Samples (4)
Array (1)
Protocols (8)
Description
modENCODE_submission_2995 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: 3rd Instar Larvae; Genotype: wild type; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody H3K18Ac (new lot) (target is H3K18Ac); Developmental Stage 3rd Instar Larvae
Experiment type
ChIP-chip by tiling array 
Contacts
DCC modENCODE <help@modencode.org>, Aki Minoda, Andrey Gortchakov, Art Alekseyenko, Cameron Kennedy, Gary Karpen, Greg Shanower, Michael Tolstorukov, Mitzi Kuroda, Nicole Riddle, Peter Kharchenko, Peter Park, Sarah Elgin, Vincenzo Pirrotta, Yuri Schwartz
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32733.idf.txt
Sample and data relationshipE-GEOD-32733.sdrf.txt
Raw data (1)E-GEOD-32733.raw.1.zip
Array designA-AFFY-146.adf.txt
R ExpressionSetE-GEOD-32733.eSet.r
Links