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E-GEOD-32523 - shRNA knockdown of ZMIZ1 in human T-cell Acute Lymphoblastic Leukemia cell line CEM

Status
Released on 13 December 2012, last updated on 2 June 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (6)
Description
Human T-cell Acute lymphoblastic Leukemia cell line CEM was transfected with either shRNA against ZMIZ1 or scrambled shRNA. Four (non-paired) biological replicates of each condition had mRNA assays performed using Affymetrix HG_U133_plus_2 arrays, with 54675 probe-sets. A supplementary Excel workbook holding the same processed data as the series matrix file is provided, with some probe set annotation, and a simple statistical comparison. The raw (.CEL) files are also provided. Keywords: Expression profiling by array Human T-cell Acute lymphoblastic Leukemia cell line CEM was transfected with either shRNA against ZMIZ1 or scrambled shRNA. Four (non-paired) biological replicates of each condition had mRNA assays performed using Affymetrix HG_U133_plus_2 arrays, with 54675 probe-sets.
Experiment type
transcription profiling by array 
Contacts
Rork Kuick <rork@umich.edu>, Derek D Garagiola, Ivan Maillard, Lesley A Rakowski, Mark Y Chiang, Mignon Loh, Morgan Jones, Rork D Kuick, Sarah C Caruso
Citation
Convergence of the ZMIZ1 and NOTCH1 pathways at C-MYC in acute T lymphoblastic leukemias. Rakowski LA, Garagiola DD, Li CM, Decker M, Caruso S, Jones M, Kuick R, Cierpicki T, Maillard I, Chiang MY. , PMID:23161489
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32523.idf.txt
Sample and data relationshipE-GEOD-32523.sdrf.txt
Raw data (1)E-GEOD-32523.raw.1.zip
Processed data (1)E-GEOD-32523.processed.1.zip
Additional data (1)E-GEOD-32523.additional.1.zip
Array designA-AFFY-44.adf.txt
Links